Fig. 4.—

Dimorphic diversity of V1R1 in the tribe Tropheini. (A) Alignment of amino acid sequences of V1R1 in 17 cichlids of tribe Tropheini (12 species). Only variable sites are shown. A dot indicates a site identical to the top sequence (Tropheus duboisi). The phase of each heterozygous V1R1 allele in the individuals is indicated in parentheses. The heterozygous at the synonymous sites are indicated as (1, 2). The sequences were divided into two major allele groups (clade I and clade II) based on the variable sites. Abbreviations of species names are listed in supplementary table S1A, Supplementary Material online. The ID number of each cichlid sample is indicated after the species name. (B) ML tree of V1R1 in cichlid tribe Tropheini. The ML tree was constructed under the GTR+I model, based on an alignment of 25 sequences of V1R1 in cichlid tribe Tropheini. The tree was rooted with Astatotilapia burtoni. Bootstrap values more than 60 are indicated above each node. The number of nonsynonymous and synonymous substitutions on the branch is indicated by ns:s.