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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Int J Biochem Cell Biol. 2014 Mar 22;51:10–18. doi: 10.1016/j.biocel.2014.03.008

Fig. 4. Aptamer #1-62 specifically recognizes CD4-positive cells in a cell mixture.

Fig. 4

Fig. 4

(A) Immunostaining of a mixture of CA46 (CD4-negative) and Sup-T1 (CD4-positive) cells with PE-labeled CD4 antibody was compared with Cy5-labeled aptamer #1-62. Staining with aptamer #1-62 was very similar to immunostaining with the CD4 antibody. (B) SupT1 cells (1 × 105) were mixed with 0.5 μL whole human peripheral blood, and the mixture was double stained with PE-CD4 antibody and cy5-labeled aptamer #1-62. Aptamer #1-62 readily bound to CD4-positive cells and was nearly indistinguishable from CD4 antibody staining.