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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: J Immunol. 2011 Jan 12;186(4):1943–1950. doi: 10.4049/jimmunol.1002422

Figure 7.

Figure 7

APE2-deficient B cell progenitors differentiate normally but expand poorly in vitro. (A) The indicated progenitor populations were purified by FACS and co-cultured with stromal cells, IL-7 and Flt3L. Sorted HSPC, MPP and CLP input cells were negative for CD19 and Mac-1. The percent of non-stromal cells expressing CD19 or Mac-1/Gr-1 (myeloid) was determined by flow cytometric analysis of cultures after 7 days. FACS plots are representative of results from two independent sorts. (B) 2,000 FACS-purified pro-B cells from WT, apex2Y/− (APE2), p53−/− (p53) or apex2Y/− p53−/−(DKO) bone marrow were cultured as in (A) and the number of viable cells was counted after 6 and 9 days. CD19 and CD43 expression on the expanded cells was confirmed by flow cytometry. The average of 3 replicate wells is shown. Data on WT and APE2-deficient pro-B cells are representative of 2 independent sorting experiments. Data on p53−/− and DKO pro-B cells are from one sort.