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Parameters for binding of ligands to ferrous ApPgb at 25°C and pH 7.0. (A) Association rates for binding of CO (triangles) and NO (circles), as determined by laser-flash photolysis. (B) Dissociation rate of CO, as determined by oxidation of CO-saturated protein with potassium ferricyanide. (C) Autoxidation of ApPgb on exposure of the deoxy form to air. Details are in Materials and Methods.
