Table 2.
Results of separate ANOVAs conducted on each protein spot detected.
| Spot N° | Dependent variables | Acc. N° | Biological activity | F | P (ANOVA) |
|---|---|---|---|---|---|
| 657 |
Indolethylamine N-methyltransferase |
gi|731019 |
Methylation, ageing |
7.80 |
0.011 |
| 516 |
Fructose-1,6-biphosphatase 1 |
gi|14547989 |
Carbohydrate metabolism, calcium chelation |
7.58 |
0.012 |
| 570 |
Glycine N-methytransferase |
gi|15679953 |
Biogenesis, methylation, carbohydrate metabolism |
6.12 |
0.021 |
| Malate dehydrogenase 1 |
gi|148675904 |
||||
| 422 |
Alpha-enolase |
gi|13637776 |
Cell growth, biogenesis, methylation |
5.19 |
0.032 |
| Adenosylhomocysteinase |
gi|21431841 |
||||
| 553 |
Dimethylarginine dimethylaminohydrolase 1 |
gi|45476974 |
Regulation of nitric oxide generation |
4.83 |
0.038 |
| 383 |
Selenium-binding protein 1 |
gi|148840436 |
Cell differentiation |
4.51 |
0.044 |
| 748 |
Regucalcin |
gi|2498920 |
Calcium homeostasis, ageing |
4.46 |
0.045 |
| 355/365 |
Catalase |
gi|157951741 |
Response to oxidative stress |
0.01/0.03 |
0.99/0.97 |
| 437 |
Ndufs 2 protein |
gi|13278096 |
Response to oxidative stress |
0.08 |
0.93 |
| 637 |
Hydroxyacyl glutathione hydrolase |
gi|13435786 |
Response to oxidative stress |
1.10 |
0.37 |
| Carbonic anhydrase 3 |
gi|148673185 |
||||
| 663/672 |
Peroxiredoxin 6 |
gi|6671549 |
Response to oxidative stress |
0.73/0.77 |
0.51/0.49 |
| 702/791 |
Peroxiredoxin 1 |
gi|547923 |
Response to oxidative stress |
0.73/0.38 |
0.51/0.69 |
| 843/850 |
Glutathione transferase |
gi|193703 |
Response to oxidative stress |
0.05/0.11 |
0.95/0.90 |
| 681/683 |
Glutathione transferase zeta 1 |
gi|148670978 |
Response to oxidative stress |
0.91/0.06 |
0.44/0.94 |
| 702 |
SOD 2 protein |
gi|17390379 |
Response to oxidative stress |
0.73 |
0.51 |
| 665/853 |
Glutathione S transferase Mu 1 |
gi|121716 |
Response to oxidative stress |
0.17/0.42 |
0.85/0.67 |
| 665/673 |
Glutathione S transferase Mu 2 |
gi|121718 |
Response to oxidative stress |
0.17/0.80 |
0.85/0.48 |
| 853 |
Glutathione S transferase Mu 3 |
gi|121720 |
Response to oxidative stress |
0.42 |
0.67 |
| 850 |
Glutathione S transferase Kappa 1 |
gi|47116757 |
Response to oxidative stress |
0.11 |
0.90 |
| 680 |
Glutathione S transferase theta-1 |
gi|160298219 |
Response to oxidative stress |
0.68 |
0.53 |
| 791 |
Glutathione S-transferase P 1 |
gi|121747 |
Response to oxidative stress |
0.38 |
0.69 |
| 681 |
Thioredoxin-dependent peroxide reductase |
gi|126986 |
Response to oxidative stress |
0.91 |
0.44 |
| 639 |
Carbonic anhydrase 2 |
gi|146345383 |
Response to oxidative stress |
0.80 |
0.48 |
| 386/390/393/633/637/647/649/778 | Carbonic anhydrase 3 | gi|148673185 | Response to oxidative stress | 0.22/0.07/0.19/0.29/1.10/0.10/0.58/0.36 | 0.81/0.93/0.83/0.76/0.37/0.90/0.58/0.71 |
Data were collected in reproductive females belonging to FEL, FRL and Control groups (n = 4 per group). Multiple ANOVAs were used to determine the spots that were likely to be affected by the experimental treatment, and that then entered the subsequent statistical analysis (see Materials and methods for details). Only 7 protein spots containing 9 different proteins were found to be significantly affected by the litter manipulation (in bold). The proteins related to oxidative balance that were detected in the proteomic analysis of the liver samples but which were apparently not affected by litter size are also indicated. Spot N° correspond to the protein spots reported in Additional file 1: Figure S1. The biological activity of proteins as determined using the so-called biological process ontologies (http://www.geneontology.org/) were automatically extracted using the MSDA software suite (https://msda.unistra.fr), and complemented with literature examination.