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. 2014 May 20;21(1):49. doi: 10.1186/1423-0127-21-49

Figure 3.

Figure 3

The phosphorylation of p38 MAPK in visfatin-induced MUC8 and MUC5B expression in NCI-H292 cells. (A) Western blot analysis showed that visfatin activated phosphorylation of p38 MAPK; however, visfatin did not activate phosphorylation of ERK1/2 MAPK. (B) RT-PCR showed that SB203580 inhibited visfatin-induced MUC8 and MUC5B expression; however, U0126 did not inhibit visfatin-induced MUC8 and MUC5B expression. (C) RT-PCR showed the knockdown of p38 MAPK by siRNA significantly blocked visfatin-induced MUC8 and MUC5B mRNA expression. Images are representative of three separate experiments performed in triplicate. Bars indicate the mean ± S.D. of three independent experiments performed in triplicate. *P < 0.05 compared with zero value. **P < 0.05 compared with visfatin alone, †P < 0.05 compared with negative control.