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. 2014 May 20;21(1):49. doi: 10.1186/1423-0127-21-49

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Copyright © 2014 Song et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The role of ROS in visfatin-activated phosphorylation of p38 MAPK in NCI-H292 cells. (A) Western blot showed that both NAC and DPI did not attenuate visfatin-activated phosphorylation of p38 MAPK. (B) Flow cytometry showed that SB203580 significantly inhibited visfatin-induced ROS formation. Images are representative of three separate experiments performed in triplicate. Bars indicate the mean ± S.D. of three independent experiments performed in triplicate. *P < 0.05 compared with zero value. **P < 0.05 compared with visfatin alone.