Skip to main content
. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: Cell. 2013 Oct 3;155(2):285–295. doi: 10.1016/j.cell.2013.08.057

Figure 5. PAR2 activation promotes itch behaviors and Ca2+-dependent release of TSLP.

Figure 5

(A) Itch-evoked scratching following injection of tryptase into the cheek (100 pg/20 μL) of wild type (WT; black), PAR2-deficient (blue) or IL7Rα-deficient mice (red), or PBS (white, 20μL) injection into WT mice, n≥8 mice per condition. The time spent scratching was quantified for 1 h after injection. (B) TSLP secretion evoked by 24 h treatment with vehicle (VEH), tryptase (TRY, 100nM), tryptase in the absence of extracellular Ca2+ (TRY 0Ca), SLIGRL (100μM), SLIGRL in the absence of extracellular Ca2+ (SLIGRL 0Ca), or TG (1μM). n≥4 replicates/condition *P<0.05; **P<0.01;, ***P<0.001. Error bars represent s.e.m.