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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Microbiol Methods. 2014 Mar 13;100:111–120. doi: 10.1016/j.mimet.2014.02.020

Figure 2.

Figure 2

Four-slice images containing bacteria and surface components. A) Test Image 1 of connected-biofilm bacteria (B* and B) grown on top of an irregular surface (S). The indexed bacteria that were used for the modified substratum calculation (Equation 2) are depicted as B*. B) Binary image sequence of the surface from Test Image 1. C) Binary image sequence of the connected-biofilm bacteria from Test Image 1. D) Binary image sequence of the indexed bacteria B*.