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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Microbiol Methods. 2014 Mar 13;100:111–120. doi: 10.1016/j.mimet.2014.02.020

Figure 4.

Figure 4

A) Original confocal image of green-fluorescent Pseudomonas aeruginosa bacteria grown on red membrane-stained human bronchial epithelial cells (16HBEo-). B) Connected-biofilm bacteria quantified with connected volume filtration (CVF). C) Connected-biofilm bacteria quantified with modified connected volume filtration (MCVF). D) Modified substratum coverage of index bacteria on cells. Top: aerial view with scale bar of 80 μm. Bottom: side view with side of square equal to 42.7 μm. Threshold values were 149 for surface and 43 for bacteria.