Fig. 2.

BRL restores NOS activity and suppresses hypertrophy-induced superoxide generation via a nNOS-dependent manner in hypertrophied cardiomyocytes. Cells were stimulated for 45 min with BRL in the presence or absence of 72 h NE pretreatment, or LVNIO, nNOS inhibitor. Hypertrophied cells were stimulated at indicated time points to measure A.) NOS activity, B.) superoxide generation in the absence or C–D.) presence of LVNIO. NOS activity was measured by 14C arginine to citrulline conversion and superoxide was measured by Lucigenin Assay. Data is presented as mean ± SEM for 3–6 experiments. *p < 0.001 vs. hypertrophied control; #p < 0.05 vs. LVNIO treated.