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BRL alters phosphorylation of nNOS and eNOS in hypertrophied cardiomyocytes. Cells were stimulated for 45 min with BRL in the presence or absence of 72 h NE pretreatment. Cells were stimulated at indicated time points to measure A.) nNOS-Ser1412 and -Ser847, and B.) eNOS-Ser1177 and -Ser114 phosphorylations. Phosphorylation was measured by Immunoblot analysis with phosphospecific antibodies. Data is presented as mean ± SEM for 3–6 experiments. *p < 0.05 vs. basal.
