Fig. 5.

nNOS phosphomimetic mutants alter BRL-induced NOS activity and superoxide suppression in hypertrophied cardiomyocytes. Hypertrophied cells were infected with Myc-tagged Sindbis viruses encoding an aspartate (D) or alanine (A) site-mutation for Ser847 or Ser1412 for 24 h prior to 45 min BRL treatment. A.) Infection was determined using Immunoblot analysis with antibodies against Myc protein. B.) NOS activity was measured by 14C arginine to citrulline conversion and C.) superoxide was measured by Lucigenin Assay. GFP Sindbis virus was used to define basal levels of transformation. Data is presented as mean ± SEM for 3–6 experiments. *p < 0.05 vs. respective viral control. #p < 0.05 vs. GFP baseline.