Fig. 6.

Akt inhibition blocks β3-AR-induced NOS activity and Ser1412 phosphorylation in hypertrophied cardiomyocytes. Cells were stimulated for 45 min with BRL in the presence or absence of Akt-i, Akt inhibitor, after NE pretreatment. Phosphorylation, NOS activity and superoxide were measured by A–B.) immunoblotting, C.) 14C arginine to citrulline conversion, and D.) lucigenin assay, respectively. E.) Left ventricular myocardium was homogenized and immunoprecipitated with anti-nNOS then probed with anti-Akt. Data is presented as mean ± SEM for three experiments. p < 0.05 vs. basal; #p < 0.05 vs. Akt-i + BRL group.