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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: J Mol Cell Cardiol. 2013 May 2;62:8–17. doi: 10.1016/j.yjmcc.2013.04.025

Fig. 7.

Fig. 7

Gi/o inhibition blocks β3-induced Ser1412 phosphorylation, NOS activity, and superoxide suppression while partially restoring Ser847. Cells were pretreated with NE for 72 h and stimulated for 45 min with BRL in the presence or absence of Pertussis Toxin, Gi/o inhibitor. Phosphorylation was detected with A.) Ser1412 and B) nNOS-Ser847 phospho-specific antibodies. C.) NOS activity and D.) superoxide generation were measured by 14C arginine to citrulline conversion and Lucigenin assay, respectively. Data is presented as mean ± SEM for three experiments. *p < 0.05 vs. basal; #p < 0.05 vs. BRL alone.