Table 1. Kinetic parameters for the binding of tau* to taxol-stabilized microtubules.
Parameter† | Optimized value‡ |
---|---|
k1 | 294 ± 18 μM−1·s−1 |
k2 | 2.5 ± 0.9 s−1 |
k3 | 35 ± 3 s−1 |
k4 | 31 ± 4 s−1 |
N§ | 0.29 ± 0.003 |
KD¶ | 16 ± 5 nM |
Refer to scheme 3 of Fig. 2C.
Values are the mean of two independent experiments; errors are propagated from standard deviation values (n = number of data points) derived from each experiment.
Maximal stoichiometry of tau:tubulin dimer.
Calculated: KD = (k2/k1)(1 + k4/k3).