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Two possible mechanisms for clustering COPII vesicles at tER sites.
A: According to the tER scaffold model, a protein such as Sec16 resides at the ER surface, where it recruits and/or crosslinks COPII components. B: According to the tER-Golgi tethering model, proteins such as p115/Uso1 link COPII components to adjacent early Golgi or pre-Golgi membranes.
