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. 2004 Apr 16;101(17):6780–6785. doi: 10.1073/pnas.0305905101

Fig. 1.

Fig. 1.

Binding of ADSF-hFc to ADSF. 3T3-L1 cells were differentiated in conditioned media from COS7 cells transfected with pcDNA3.1 control vector or ADSF expression vectors. (A) Conditioned media containing ADSF-HA, ADSF-hFc, or both ADSF-HA and ADSF-hFc were subjected to reducing (15%) or nonreducing (6%) SDS/PAGE followed by Western blot analysis with antibodies against ADSF, HA, or human Fc fragment. (B) Conditioned media containing ADSF-HA or ADSF-HA and ADSF-hFc were immunoprecipitated by using anti-hFc agarose beads followed by Western blot analysis with antibodies against ADSF and HA.