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Effect of conditioned media from COS7 cells transfected with ADSFHA, ADSF-hFc, or ADSF-HA and ADSF-hFc on 3T3-L1 adipocyte differentiation. (A) Microscopic pictures for cell morphology and Oil red O staining of 3T3-L1 cells after subjection to differentiation protocol. (B) Adipocyte markers (PPARγ and aFABP/aP2) and a preadipocyte marker (Pref-1) were analyzed by RT-PCR by using total RNA isolated from conditioned-medium-treated 3T3-L1 adipocytes. β-Actin was used as control.
