Fig. 1.

(A) Bar diagram representing the viroid sequences embedded in the transcripts expressed in the transgenic Arabidopsis. Viroid species are indicated on the left, and the database accession numbers of the particular sequence variants used are on the right. Whenever sequence variants do not exactly match a database entry, the differences are indicated in parentheses after the accession number. Positions where the viroid sequences start and end in the transcript and the size of each monomeric unit are also indicated. (B) Dot-blot hybridization analysis of transgenic Arabidopsis expressing different dimeric (+) viroid RNAs. Total RNAs from three independent transgenic lines (columns 1, 2, and 3) for each of the six constructs (CEVd, HSVd, CCCVd, ASSVd, CbVd-1, and ASBVd) were hybridized with each of the six ³²P-labeled complementary riboprobes indicated on the left.