Table 1. Sequence of monomeric circular (+) RNAs accumulating in transgenic Arabidopsis expressing viroid dimeric (+) RNAs.
Viroid species | Primers* | Clones sequenced | Observed mutations |
---|---|---|---|
CEVd | 75-95, 96-113 | 10 | Two clones: C38→U, G287→U |
HSVd | 64-87, 88-109 | 6 | None |
276-1, 2-23 | 6 | Two clones: +A(39-45) | |
CCCVd | 41-65, 66-90 | 9 | None |
ASSVd | 79-89, 90-110 | 10 | One clone: G29→Δ, C31→U, C210→U |
ASBVd | 42-57, 58-78 | 10 | One clone: U148→C, C151→Δ |
One clone: G196→A |
Positions of the viroid sequence covered by primers of (−) and (+) polarities, respectively, used for RT-PCR amplification.