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. Author manuscript; available in PMC: 2015 Jan 20.

Published in final edited form as: Angew Chem Int Ed Engl. 2013 Dec 11;53(4):1140–1143. doi: 10.1002/anie.201308607

Binding of probes to DD(E) (2 μM) is detected by measuring the decrease in fluorescence anisotropy that occurs upon competitive displacement of the TRITC-Tn6 probe (0.1 μM). A) EP-2104R (○) exhibits a strong dose-dependent response whereas 1 (●) does not. B) The conversion of 1 (10 μM) to EP-2104R is followed in real-time by observing the displacement of TRITC-Tn6 from DD(E). The reaction was run under four different reaction conditions: untreated (○); 1 mg/L PDI (△); 10 μM DTT (●); and 10 μM DTT plus 1 mg/L PDI (◆).

Binding of probes to DD(E) (2 μM) is detected by measuring the decrease in fluorescence anisotropy that occurs upon competitive displacement of the TRITC-Tn6 probe (0.1 μM). A) EP-2104R (○) exhibits a strong dose-dependent response whereas 1 (●) does not. B) The conversion of 1 (10 μM) to EP-2104R is followed in real-time by observing the displacement of TRITC-Tn6 from DD(E). The reaction was run under four different reaction conditions: untreated (○); 1 mg/L PDI (△); 10 μM DTT (●); and 10 μM DTT plus 1 mg/L PDI (◆).