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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: Cancer Gene Ther. 2010 Jun 11;17(10):694–699. doi: 10.1038/cgt.2010.25

Figure 1. siRNAs specific for MCM7 inhibit MCM7 expression in PC3 cells.

Figure 1

(A) Diagram of MCM7 genome and siRNAs corresponding to the regions of MCM7. Exons are indicated by box. miRNA-106b-25 is indicated by a diamond. Introns are indicated by line. (B) Immunoblot analysis of MCM7 expression inhibition by siRNA specific for MCM7. PC3 (lanes 1-6) or Du145 (lanes 7-11) cells were transfected with siScramble (lanes 2 and 8), siMCM7α1 (lanes 4 and 10), siMCM7α2 (lanes 5 and 11) and siMCM7α3 (lanes 6 and 9) or combination of all three siMCM7 (lane 3). Immunoblot analysis were subsequently performed on the protein extracts from these cells using antibody specific for MCM7 (upper panel) or β-actin (lower panel). (C) Immunoblot analysis of MCM7 expression inhibition by pENTR-siMCM7. PC3 (lanes 1-4) or Du145 cells (lanes 5-8) were transfected with pENTR (lanes 2), pENTR-siScramble (lanes 3 and 6), pENTR-siMCM7 (lanes 4 and 8), and siMCM7α1 (lane 7). Immunoblot analysis were subsequently performed on the protein extracts from these cells using antibody specific for MCM7 (upper panel) or β-actin (lower panel).