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. Author manuscript; available in PMC: 2014 Jun 2.

Published in final edited form as: Cancer Gene Ther. 2010 Jun 11;17(10):694–699. doi: 10.1038/cgt.2010.25

(A) Inhibition of Bromo-deoxy-uridine incorporation into DNA by siRNA specific for MCM7. PC3 (left) or Du145 cells (right) transfected with siRNA for scramble (), siMCM7α1 (), pENTR-siScramble () or pENTR-siMCM7 (), were cultured for the indicated time. The cells were then pulsed labeled with BrdU for 3 hours, and the BrdU incorporations were quantified. (B) Cell cycle arrest induced by siRNA specific for MCM7. PC3 or Du145 cells transfected with the indicated siRNAs or vectors for 24 hours. The cells were then synchronized by serum starvation. These cells were later serum stimulated and pulsed labeled with BrdU for 3 hours and stained with DAPI. FACS analyses were then performed on these cells to examine cell cycle distribution.

Inline graphic — (A) Inhibition of Bromo-deoxy-uridine incorporation into DNA by siRNA specific for MCM7. PC3 (left) or Du145 cells (right) transfected with siRNA for scramble (), siMCM7α1 (), pENTR-siScramble () or pENTR-siMCM7 (), were cultured for the indicated time. The cells were then pulsed labeled with BrdU for 3 hours, and the BrdU incorporations were quantified. (B) Cell cycle arrest induced by siRNA specific for MCM7. PC3 or Du145 cells transfected with the indicated siRNAs or vectors for 24 hours. The cells were then synchronized by serum starvation. These cells were later serum stimulated and pulsed labeled with BrdU for 3 hours and stained with DAPI. FACS analyses were then performed on these cells to examine cell cycle distribution.