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. 2013 Nov 14;69(2):208–215. doi: 10.1111/all.12306

Figure 4.

Figure 4

IgG1 antibody responses at day 49 analyzed by ELISA. IgG1 levels of each variant against itself and the WT, compared with the IgG1 response of the respective WT protein. The y-axis shows Δ preserum values of the serum dilution at which 50% of the maximum absorption (405 nm) was measured. Each symbol represents sera from one of the five mice immunized with each protein (A). IgE antibody responses by mouse RBL assays. 1 : 40 dilutions of sera pools from mice immunized with each of the antigens were used to passively sensitize mouse RBL cells. Recognition and cross-linking of IgE antibodies was evaluated for the homologous molecules and the WT proteins. Data are expressed as means ± SEM of% mediator release (B). ELISPOT analysis of splenocytes from immunized mice expressed as the mean of cytokine-secreting cells per 2 × 105 cells ± SEM (C). P-values were calculated using the Mann–Whitney U-test (*P <0.05; **P <0.01).