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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Sep 8;400(4):684–688. doi: 10.1016/j.bbrc.2010.08.128

Figure 3.

Figure 3

Osx binding site is located within 260 bp of Sost promoter. (A) Deletion analysis of Sost promoter reporter activated by Osx. Sost-1 kb, Sost-540 bp, Sost-260 bp, and Sost-106 bp Sost promoter reporter plasmids were constructed. Each plasmid was cotransfected with Osx expression plasmid in HEK293 cells. Luciferase activity was normalized by β-galactosidase activity. (B) The GC-rich element in Sost-260-M1 is responsible for Sost promoter reporter activation by Osx. Sost-260-M1 and Sost-260-M2 point mutants were constructed. Each plasmid was cotransfected with Osx expression plasmid in HEK293 cells. Luciferase activity was normalized by β-galactosidase activity.