Skip to main content
. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Sep 8;400(4):684–688. doi: 10.1016/j.bbrc.2010.08.128

Figure 4.

Figure 4

Osx associates with Sost promoter. (A) Osx directly binds to Sost GC-rich sequence in EMSA. In the gel shift assays, P32-labeled oligo corresponding to Sost GC-rich sequence identified within 260 bp of Sost promoter region was incubated with Osx. Baculovirus-expressed Osx was used as the protein resource. Protein–DNA complexes were resolved on a non-denaturing polyacrylamide gel and visualized by autoradiography. (B) Osx associates with native Sost promoter in ChIP assay. Calvarial cells were isolated and cultured from wild-type new born mice. Anti-Osx antibody was used for ChIP analysis, and IgG was used as a negative control. The precipitated chromatin was analyzed by quantitative real-time PCR. Primer set 1 corresponds to a segment of the Sost promoter containing GC-rich element between 106 bp and 260 bp. As a negative control, Primer set 2 covers a distal region of the Sost promoter, which does not contain GC-rich sequence.