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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Am J Obstet Gynecol. 2014 Jan 17;210(6):547.e1–547.e7. doi: 10.1016/j.ajog.2014.01.024

Figure 1. Flt-1 expression is increased in PMAs from women with preeclampsia.

Figure 1

(A) PMAs present in freshly-isolated mononuclear cells from women with preeclampsia were stained with WGA (red), which stains sialic acids in platelets and mononuclear cells and TOPRO-3 (blue), which stains nuclei. In parallel, an antibody specific for the N-terminus of Flt-1 (green) or its IgG control was used to identify Flt-1 in monocytes. (B) Freshly-isolated monocytes (including PMAs) were immediately lysed and total (soluble and membrane-bound) Flt-1 levels were determined by ELISA. The bars in this graph represent the mean ± SEM for all participants in each group, *p=0.003 compared to pregnant and nonpregnant controls.