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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Am J Obstet Gynecol. 2014 Jan 17;210(6):547.e1–547.e7. doi: 10.1016/j.ajog.2014.01.024

Figure 4. Synthesis of sFlt-1 is regulated at the transcriptional level in PMAs.

Figure 4

Thrombin-activated platelets were added to monocytes pretreated with actinomycin-D (actD) or cycloheximide (CHX) and incubated together for either 2 or 18 hours to assess sFlt-1 mRNA (A) or protein in the supernatant (B). In panel C, thrombin-activated platelets were added to monocytes pretreated with specific inhibitors against NF- κB (Bay 11-7082), MEK 1 and 2 (U0126), JNK (SP600125), or p-38 MAPK (SB203580). The bars in this graph represent the mean ± SEM of 3-5 independent experiments. *p<0.05 compared to thrombin alone (A,B) or vehicle (C).