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Refolding assay. Left: Monomeric double-labeled αS is mixed with TMAO to induce compaction and an increase in quenching efficiency, determined from TCSPC measurements (EQ(τ)= 1− τThio/τ0). Right: EQ(τ)of αSψ114E′137 (blue) or αSψ125E′130 (red) determined at varying concentrations of TMAO. Error bars represent the uncertainty in the fitted average lifetime values for each condition. Fits to raw TCSPC data are shown in Supporting Information.
