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. 2014 Mar 25;42(10):6463–6475. doi: 10.1093/nar/gku224

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Assessment of potential off-target cleavage activity of TALE-XID enzyme. (A) Alignment of seven selected potential XID homologous sites in mouse genome. TALE, XID binding sites and mismatch positions are marked in green, red and blue, respectively. (B) Table showing 6RVD-TALE-XID off-target cleavage activity in XID MEFs. Potential sites were sequenced following PCR amplification of genomic DNA derived from XID MEF in which 40% XID site disruption had been achieved by LV TALE-XID expression (as in Figure 4E).