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ZF-TF activity varies with different activation domains. p65, 2xp65, VP16 or VP64 activation domains were fused to the following ZF-TFs: ZF(OCT4+181), ZF(SOX2–88), ZF(KLF4+134) or ZF(c-MYC-17), and transcriptional activation of OCT4 (A), SOX2 (B), KLF4 (C) and c-MYC (D) by these constructs in HEK293 cells is shown. Averages from three biological replicates are plotted with error bars representing one standard deviation.
