Table 1.
Plasmids constructed in this study
| Chimera | Plasmid name | Vector backbone | Insert |
|---|---|---|---|
| pEGFP-N1-KDM4C1-708aa | pEGFP-N1 digested with NheI, XhoI | KDM4C1-708aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F1 and R1 | |
| Chimera 1 | pEGFP-N1-KDM4C-1-708-KDM4A-707-1064 aa | pEGFP-N1-KDM4C1-708aa digested with XhoI, SalI | KDM4A-707-1064 aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F3 and R3 |
| pEGFP-N1-KDM4A1-714aa | pEGFP-N1 digested with SalI, AfeI | KDM4A1-714aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F2 and R2 | |
| Chimera 2 | pEGFP-N1-KDM4A-1-714-KDM4C-695-1056 aa | pEGFP-N1-KDM4A1-714aa digested with SalI, XmaI | KDM4C-695-1056 aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F4 and R4 |
| pEGFP-N1-Hs-KDM4C1-865aa | pEGFP-N1 digested with NheI, XhoI | KDM4C1-865aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F1 and R5 | |
| Chimera 3 | pEGFP-N1-Hs-KDM4C1-865-KDM4A886-1064aa | pEGFP-N1-Hs-KDM4C1-865aa digested with XhoI, SalI | KDM4A886-1064aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F6 and R3 |
| pEGFP-N1-KDM4A1-885aa | pEGFP-N1 digested with SalI, AfeI | KDM4A1-885aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F2 and R6 | |
| Chimera 4 | pEGFP-N1-KDM4A1-885-KDM4C866-1056aa | pEGFP-N1-KDM4A1-885aa digested with SalI, XmaI | KDM4C866-1056aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F7 and R4 |
| pEGFP-N1-KDM4C1-934 | pEGFP-N1 digested with NheI, XhoI | KDM4C1-934 was amplified from pEGFP-N1-Hs-KDM4C-wt using F1 and R7 | |
| Chimera 5 | pEGFP-N1-KDM4C1-934-KDM4A935-1064aa | pEGFP-N1-KDM4C1-934 digested with XhoI, SalI | KDM4A935-1064aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F8 and R3 |
| pEGFP-N1-KDM4A1-954 | pEGFP-N1 digested with SalI, AfeI | KDM4A1-954 was amplified from pEGFP-N1-Hs-KDM4A-wt using F2 and R8 | |
| Chimera 6 | pEGFP-N1-KDM4A1-954-KDM4C955-1056aa | pEGFP-N1-KDM4A1-954 digested with SalI and XmaI | KDM4C955-1056aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F9 and R4 |
| pEGFP-N1-KDM4C-RTDF-DNLY | pEGFP-N1-KDM4C-WT | PCR mutagenesis using primers F10 and R10 | |
| pEGFP-N1-KDM4A-DNLY-RTDF | pEGFP-N1-KDM4A-WT | PCR mutagenesis using primers F11 and R11 | |
| pEGFP-N1-KDM4C-R919D | pEGFP-N1-KDM4C-WT | PCR mutagenesis using primers F12 and R12 |