|
pEGFP-N1-KDM4C1-708aa |
pEGFP-N1 digested with NheI, XhoI
|
KDM4C1-708aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F1 and R1 |
Chimera 1 |
pEGFP-N1-KDM4C-1-708-KDM4A-707-1064 aa |
pEGFP-N1-KDM4C1-708aa digested with XhoI, SalI |
KDM4A-707-1064 aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F3 and R3 |
|
pEGFP-N1-KDM4A1-714aa |
pEGFP-N1 digested with SalI, AfeI
|
KDM4A1-714aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F2 and R2 |
Chimera 2 |
pEGFP-N1-KDM4A-1-714-KDM4C-695-1056 aa |
pEGFP-N1-KDM4A1-714aa digested with SalI, XmaI |
KDM4C-695-1056 aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F4 and R4 |
|
pEGFP-N1-Hs-KDM4C1-865aa |
pEGFP-N1 digested with NheI, XhoI
|
KDM4C1-865aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F1 and R5 |
Chimera 3 |
pEGFP-N1-Hs-KDM4C1-865-KDM4A886-1064aa |
pEGFP-N1-Hs-KDM4C1-865aa digested with XhoI, SalI |
KDM4A886-1064aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F6 and R3 |
|
pEGFP-N1-KDM4A1-885aa |
pEGFP-N1 digested with SalI, AfeI
|
KDM4A1-885aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F2 and R6 |
Chimera 4 |
pEGFP-N1-KDM4A1-885-KDM4C866-1056aa |
pEGFP-N1-KDM4A1-885aa digested with SalI, XmaI |
KDM4C866-1056aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F7 and R4 |
|
pEGFP-N1-KDM4C1-934 |
pEGFP-N1 digested with NheI, XhoI
|
KDM4C1-934 was amplified from pEGFP-N1-Hs-KDM4C-wt using F1 and R7 |
Chimera 5 |
pEGFP-N1-KDM4C1-934-KDM4A935-1064aa |
pEGFP-N1-KDM4C1-934 digested with XhoI, SalI
|
KDM4A935-1064aa was amplified from pEGFP-N1-Hs-KDM4A-wt using F8 and R3 |
|
pEGFP-N1-KDM4A1-954 |
pEGFP-N1 digested with SalI, AfeI
|
KDM4A1-954 was amplified from pEGFP-N1-Hs-KDM4A-wt using F2 and R8 |
Chimera 6 |
pEGFP-N1-KDM4A1-954-KDM4C955-1056aa |
pEGFP-N1-KDM4A1-954 digested with SalI and XmaI
|
KDM4C955-1056aa was amplified from pEGFP-N1-Hs-KDM4C-wt using F9 and R4 |
|
pEGFP-N1-KDM4C-RTDF-DNLY |
pEGFP-N1-KDM4C-WT |
PCR mutagenesis using primers F10 and R10 |
|
pEGFP-N1-KDM4A-DNLY-RTDF |
pEGFP-N1-KDM4A-WT |
PCR mutagenesis using primers F11 and R11 |
|
pEGFP-N1-KDM4C-R919D |
pEGFP-N1-KDM4C-WT |
PCR mutagenesis using primers F12 and R12 |