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. 2014 Apr 7;42(10):6183–6195. doi: 10.1093/nar/gku261

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Expression of methionine synthase (MET-8) is decreased in cul4^KO and ddb1^KO strains. (A) Total protein extracts from N. crassa wild-type (WT) cells were separated by 2D gel electrophoresis. The rectangle shows the location of MET-8 proteins in the 2D map. (B) Expression pattern of MET-8 proteins in WT, cul4^KO and ddb1^KO strains. The arrows indicate the spots of MET-8 proteins identified by LC–MS/MS. (C) Identification of MET-8 proteins in N. crassa using LS-MS/MS. E: experimental values obtained directly from gels; T: theoretical values obtained from ExPASy search for full-length precursor proteins; MW: molecular weight (kDa). (D) Protein spots of MET-8 in 2D gels were confirmed by western blot analysis using antiserum against MET-8. Five protein spots were found on a PVDF membrane (top panel) containing proteins transferred from a 2D gel with WT sample (bottom panel) stained by CBB R-350.