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. 2014 Apr 7;42(10):6183–6195. doi: 10.1093/nar/gku261

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Localization and organization of the met-8 gene on linkage group III of N. crassa. (A) and (B) Schematic depicting a 50-kb AT-rich DNA region located between met-8 and NCU06171 on linkage group III of N. crassa genome. Bars (primer pairs 1–5) above the schematic indicate the regions tested by qPCR in ChIP assays. (C)–(G) ChIP analyses showing enrichment of H3K9 trimethylation at different regions upstream of met-8 in wild-type, cul4^KO, ddb1^KO, dcaf26^KO, dim-7^KO, dim-5^KO and hpo^KO strains. The loci detected by ChIP are indicated in (B). (H) and (I) Northern blot analysis showing levels of NCU06171 and NCU06511 mRNA in wild-type, ku70^RIP, cul4^KO, ddb1^KO, dcaf26^KO, dim-7^KO, dim-5^KO and hpo^KO strains.