Figure 2.
Editing set-up to study the influence of the 5′-neighbor: the XAG codon (X = U, A, C, G) in GFP mRNA (dark blue) was targeted by SNAP-ADAR/guideRNA conjugates in a way that the targeted adenosine (A*) was either base paired with U or mismatched with C, whereas the 5′-neighboring base X was either base paired or mismatched in all possible combinations. Both deaminase domains of hADAR1 and hADAR2 were studied.