Figure 3.

Control of editing selectivity by guanosine mismatching. Mismatching an adenosine base with guanosine completely inhibits editing. This can be exploited to block unwanted off-site editing of the functionally important Tyr66 codon (UAU) in GFP mRNA. G-mismatching allows for the highly selective editing of Ser67 (U AGC) and Tyr65 (UAC) missense mutations in GFP. However, the latter is only efficiently edited with SNAP-ADAR1. The guideRNA is shown in red, the mRNA in blue and the targeted adenosine is marked by an asterisk.