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Selection of sequencing results for the codons XA*G, X = U, A, C, G. For every codon, the optimal guideRNA architecture is given first, followed by suboptimal architectures that show the necessity for matching (UAG) or mismatching at the 5′-neighbor X (CAG and GAG) and a notable dependence on magnesium (CAG). Except the three marked examples all editings were performed in presence of 0.75 mM magnesium.
