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. 2014 May 5;42(10):6326–6336. doi: 10.1093/nar/gku276

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited

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Figure 2. — hSSB1 is localized to stalled replication forks. (A) hSSB1 is loaded onto chromatin following HU treatment. HeLa cells were treated or mock-treated with 2 mM HU for the indicated time before protein fractionations were carried out. (B) hSSB1 co-localizes with BrdU at stalled replication forks. HeLa cells were labelled with BrdU before treatment with 2 mM HU. Cells were then fixed and stained with the indicated antibodies. (C) hSSB1 partially co-localizes with other proteins required for repair of stalled replication forks. HeLa cells were treated with 2 mM HU for 20 h, fixed and stained with the indicated antibodies. (D) hSSB1 co-localizes with RPA34 after replication fork stalling. HeLa cells were treated with 2 mM HU for the indicated time and stained with the indicated antibodies.