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. 2014 May 5;42(10):6326–6336. doi: 10.1093/nar/gku276

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited

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Figure 6. — Defective recruitment of DNA repair proteins to collapsed replication forks in hSSB1-deficient cells. (A), (B) HeLa cells were transfected with control or hSSB1 siRNA and treated or mock-treated with 2 mM HU. Cells were fixed and stained with (A) p-RPA34 S4/8 or (B) Rad51. Cells were scored as being positive (>10 foci) or negative for foci. Representative images and the means and s.d. (bars) of three independent experiments are shown. (C) HeLa cells were transfected with mock or hSSB1 siRNA, treated with 2 mM HU and subcellular fractionations performed. Immunoblotting of the nuclear and chromatin fractions was carried out with the indicated antibodies. *P = 0.0043, **P = 0.032.