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RalR cleaves DNA. (A) RalR cleaves lambda DNA after 30 min and 120 min. (B) RalR cleaves unmethylated lambda DNA (dam−, dcm−) after 30 min and 120 min. In (A) and (B), the positive control is degradation of DNA by DNase I, and the negative control is the inactive RalR-mutant (RalR K52Q). EDTA blocks RalR and DNase I activity. (C) RalR activity requires co-factor Mg2+ and/or Ca2+.
