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. 2014 Apr 19;42(10):6448–6462. doi: 10.1093/nar/gku279

Figure 3.

Figure 3.

RalR is a protein and RalA functions as RNA. (A) Upper panel (SDS-PAGE) and lower panel (western blot) show that there is less RalR protein in the presence of RalA. His-tagged RalR (marked with arrows) was produced from pCA24N-ralR and pCA24N-ralR-ralA and has six histidine residues and 10 other amino acids attached at the N-terminus (expected size of ∼9.05 kDa). Production of RalR and RalA was induced via 0.2 mM IPTG at a turbidity of 0.1 for 5 h, and no IPTG treatment was used for the negative control. (B) Cell growth on LB plates with 0.5 mM IPTG in the Δrac host. T189C refers to the ydaC mutation in which the putative start codon ATG at position 189 was mutated into ACG, and C161A refers to the ralA mutation containing a stop codon instead of the ninth codon of putative protein YdaC.