Table 2.
Levels of RalR and RalA transcripts (Ct values ± S.D.) quantified by qRT-PCR in BW25113/pBAD-ralA
| Conditions | Ct (target gene) | Ct (control) | Fold change |
|---|---|---|---|
| ralR | rrsG | ||
| 20 min − ara | 28 .0 ± 0.1 | 8.9 ± 0.2 | 1.7 ± 0.2 (ralR) |
| 20 min + ara | 27.0 ± 0.2 | 8.7 ± 0.1 | |
| 8 h − ara | 29.8 ± 0.2 | 8.9 ± 0.0 | 1.5 ± 0.4 (ralR) |
| 8 h + ara | 29.0 ± 0.4 | 8.7 ± 0.1 | |
| ralA | rrsG | ||
| 20 min − ara | 19.7 ± 0.6 | 8.9 ± 0.2 | 22.6 ± 0.2 (ralA) |
| 20 min + ara | 15.0 ± 0.4 | 8.7 ± 0.1 | |
| 8 h − ara | 20.3 ± 0.1 | 8.9 ± 0.0 | 11.3 ± 0.1 (ralA) |
| 8 h + ara | 16.6 ± 0.1 | 8.7 ± 0.1 |
Exponentially growing cells (OD600 0.8) were induced with 1% L-arabinose (+ ara) or without L-arabinose (− ara) for 20 min and 8 h. Lower Ct values indicate higher expression levels, and rrsG was used to normalize total RNA levels. Two independent cultures were used for the assay. Fold changes in the transcription of various targets with or without L-arabinose were calculated as 2∧−(Cttarget(+ ara)−Ct rrsG(+ ara))/2∧−(Cttarget(− ara)−CtrrsG(− ara)). Means and standard errors are indicated.