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. 2014 Apr 19;42(10):6603–6615. doi: 10.1093/nar/gku286

Figure 6.

Figure 6.

U1C depletion decreases the efficiency of cis-splicing. (A) Schematic overview of the primer combinations used to detect PAP [poly(A) polymerase, Tb927.3.3160] pre-mRNA and mRNA by combinations of exon-, intergenic-region, intron- or SL-specific primers. The same primer pairs detected both cis-spliced and cis-unspliced products, as well as trans-spliced mRNA (mRNA) and aberrant trans-splicing at exon 2 (trans-Ex2). (B) Inhibition of cis-splicing by U1C knockdown. Total RNA from uninduced (−) and induced (+) cells after 72 h were analyzed by semiquantitative RT-PCR, using the primer combinations described in panel (A). As a control, U3 RNA was measured from the same RNA samples. M, markers (100, 200, 300, 400, 500, 600 and 700 bp). (C) Quantification of RT-PCR reactions shown in panel (B). Error bars represent standard deviations from three independent experiments. *P < 0.05 and **P < 0.01 versus uninduced control.