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. 2014 Apr 20;42(10):6352–6364. doi: 10.1093/nar/gku295

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited

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Figure 4. — Intragenic deletions in Notch1 and Bcl11b. Genomic DNA from thymocytes of indicated genotypes was used in nested PCR to detect a 12 and 6 5kb deletions in Notch1 and Bcl11b genes, respectively. (A) Comparison between WT and RAG^FS/FS mice. *P < 0.03, **P < 0.003. Sequence analysis of the PCR products for Notch1 (B) and Bcl11b (C). First line represents the germline sequence with bold letters indicating the cRSS from each side. Capital letters in the middle of the junction are N nt. Bold capital letters are P nt. WT, n = 1; RAG2^FS/FS, n = 2–4.