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. 2014 Apr 20;42(10):6365–6379. doi: 10.1093/nar/gku296

Figure 7.

Figure 7.

Maintenance of γH2AX foci requires NuMA. (a–b) Accumulation of γH2AX in S1 cells transfected with NuMA-specific or with nontargeting siRNA. The cells were irradiated (3 Gy) and left to recover for 30 min, 2 h and 6 h before immunostaining for γH2AX and NuMA. (a) Representative confocal images. (b) Quantification of foci densities. Cells treated with NuMA siRNA that retained NuMA expression were excluded from the analysis. Values normalized to the nontarget siRNA transfection are shown in the inset (n = 4; *P < 0.05, one-sample t-test). (c) Western blot analysis using antibodies against γH2AX, NuMA (to assess silencing), as well as lamin B and H2B (loading controls) of S1 cells treated as in A–B. (d) Confocal images and (e) quantification of 53BP1 foci formation in S1 cells treated as in (a–b) (n = 4). Scale bars, 10 μm.