Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 20;42(10):6405–6420. doi: 10.1093/nar/gku303

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — Female MEF single clones stably express the GFP-tagged histone variants. (A) FACS profile of female MEF cell populations derived from sorted single cell clones that express H2A.Bbd, macroH2A.1.2 and H2A tagged with GFP (solid line) in comparison to untransfected control female MEF cells (dotted and filled histogram). (B) Western blot analysis of GFP-tagged histone variant expression levels with antibody against GFP. Detection of histone H3 served as loading control. (C) Nuclear localization of the histone variants GFP-H2A.Bbd and -macroH2A.1.2 in female MEFs stained with H3K27me3 (red). Arrows indicate the Barr body. Scale bar: 5 μm.