Figure 2.

Inhibition of TAP-dependent RNA export by Rev. (A) The same ³²P-labeled RNA mixture as in Figure 1 together with ³²P-labeled m⁷G-capped U5ΔSm RNA was microinjected into the nucleus of oocytes. The effect of the wild-type Rev or Rev M10 mutant protein (160 fmol/oocyte) was examined as in Figure 1. (B) Quantitation of the export of spliced ftzRRE, elongated U1 and U5 RNAs from three independent experiments performed as in (A). Averages and standard deviations with M10 (black bars) or without proteins (buffer; white bars) are shown. (C and D) The same ³²P-labeled RNA mixture as in (A), except that either pre-CDCRRE (C) or pre-betaRRE (D) was used instead of pre-ftzRRE, was microinjected into the nucleus. The effect of the wild-type Rev or Rev M10 mutant protein (160 fmol/oocyte) was examined as in (A). See also Supplementary Figure S1 for quantitation.