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Effect of Rev on EJC formation. 32P-labeled pre-ftzRRE RNA (left) or intronless ftzRRE RNA (right) was microinjected into the nucleus in either the absence (A) or presence (B) of Rev. The nuclear fraction was prepared after 1 h, and RNase H digestion was performed with the antisense oligo DNAs A–D. RNA digestion was analyzed using 6% denaturing PAGE.
