Table 1. Synthetic complete glucose (SDC) medium.

Component	g/L
d-Glucose	20
Ammonium sulfate	5
Nitrogen base (−AS/−AA)	1.8
NaH₂P0₄	1.4
	mg/L
Adenine	80
l-Arginine	40
l-Aspartic acid	100
l-Glutamic acid	100
l-Histidine	80
l-Isoleucine	60
l-Leucine	120
l-Lysine	60
l-Methionine	80
l-Phenylalanine	60
l-Serine	400
l-Threonine	200
l-Tryptophan	80
l-Tyrosine	40
l-Valine	150
Uracil	80

SDC (18) is supplemented with a four-fold excess of histidine, leucine, tryptophan, and uracil to compensate the auxotrophies in the DBY746 strain. Similar supplementations should be carried out based on the autotrophics of the genetic background used. Adjust to pH 6.0 with NaOH.